WebTotal-BRD4 assay principle. The HTRF Total-BRD4 assay quantifies the expression level of BRD4 in a cell lysate. Unlike Western Blot, the assay is entirely plate-based and does not require gels, electrophoresis, or transfer. The Total-BRD4 assay uses two labeled antibodies: one coupled to a donor fluorophore, the other to an acceptor. WebNon-targeting pool (NTP) siRNA at 0.5 μM was used as a control. Cells were lysed for HTRF assay or fixed for immunofluorescence at DIV18. Total tau HTRF of seeded primary neurons treated with siRNA at DIV7 (B) or DIV11 (C). Accumulated tau as measured by Tau aggregation HTRF (D-E) or Triton-insoluble AT8 spots as quantified by IF (F-G). Grey ...
Phospho-Phospholamban cellular HTRF kit Cisbio
WebHTRF ® pre-mixed antibodies. Remove Lysis. Transfer 16 µL supernatant TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: Small volume detection plate 96-half well plate. 25 µL cells + 5 µL compounds + 10 µL supplemented lysis buffer (4X)* + 4 µL HTRF ® pre-mixed antibodies. Read & analyse. Incubate 2 h at RT Transfer 16 µL supernatant. … heritage npt facebook
A seeding-based neuronal model of tau aggregation for use in …
WebThis HTRF kit detects changes of cAMP accumulation in response to Gs coupled GPCR activation or inhibition. ... Plate Storage Solutions for Liquid Handling Automation Remote Lab Management ... Cisbio's cAMP kits are best suited for measuring cAMP modulations in response to Gs-activation, leveraging an optimized combination of robustness and ... WebThe HTRF total-EGFR assay is at least 15-fold more sensitive than the Western Blot and shows optimal correlation. Inhibition measured with Phospho-EGFR (tyr1068) and total EGFR cellular kits Human epidermoid carcinoma A431 cells were pre-treated for 3 h with a dose-response of Cetuximab, an extracellular therapeutic antibody, then stimulated ... WebThe SpectraMax Paradigm Cisbio HTRF Detection Cartridge is a dual emission detection cartridge and occupies two (2) slots. It may be installed in the top read detection … mauis brothers names